anti ctr  (Bioss)

Journal: Molecular Neurobiology

Article Title: Mechanistic Investigation of TSPO-Mediated Dysregulation of Mitochondrial Copper Homeostasis in Microglia and its Role in Perioperative Neurocognitive Disorders

doi: 10.1007/s12035-026-05692-4

Figure Lengend Snippet: TSPO elevation disrupts copper homeostasis in BV-2 cells. A Compare the changes in intracellular copper ion content among LPS-stimulated BV-2 cells across different groups. B Representative images of ATP7A, HSP70, CTR-1, FDX-1, and β-actin expression in LPS-stimulated BV-2 cells by western blot. C – F Comparison of ATP7A, HSP70, CTR-1, and FDX-1 expression in the LPS-stimulated BV-2 cells in each group based on western blot analysis ( n = 5). G Quantification of the fluorescence intensity of ATP7A using Image-Pro Plus ( n = 5). H Representative immunocytochemistry of ATP7A expression in the BV-2 cells. Scale bar, 20 µm. I Representative immunocytochemistry of CTR-1 expression in the BV-2 cells. Scale bar, 20 µm. J Representative immunocytochemistry of COX17 expression in the BV-2 cells. Scale bar, 20 µm. K Quantification of the fluorescence intensity of CTR-1 using Image-Pro Plus ( n = 5). L Quantification of the fluorescence intensity of COX17 using Image-Pro Plus ( n = 5). Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: CTR-1 Abmart , T510261 AB_3644314 , Rabbit 1:1000.

Techniques: Expressing, Western Blot, Comparison, Fluorescence, Immunocytochemistry

Journal: Molecular Neurobiology

Article Title: Mechanistic Investigation of TSPO-Mediated Dysregulation of Mitochondrial Copper Homeostasis in Microglia and its Role in Perioperative Neurocognitive Disorders

doi: 10.1007/s12035-026-05692-4

Figure Lengend Snippet: Disruption of copper homeostasis as a primary mechanism behind TSPO-induced mitochondrial structural and functional abnormalities. A , B , C Comparison of FDX-1 mRNA and protein expression in FDX-1 knockdown cells and the control siRNA cells ( n = 3). D Comparison of copper ion content in the LPS-stimulated BV-2 cells in each group ( n = 5). E Representative images of ATP7A and β-actin expression in LPS-stimulated BV-2 cells by western blot. F Comparison of ATP7A expression in the LPS-stimulated BV-2 cells in each group based on western blot analysis ( n = 5). G Comparison of CTR-1 expression in the LPS-stimulated BV-2 cells by group using qRT-PCR ( n = 5). H Comparison of ATP7A expression in the LPS-stimulated BV-2 cells by group using qRT-PCR ( n = 5). I Quantification of the fluorescence intensity of the ROS using Image-Pro Plus ( n = 3). J Representative images showing the ROS expression in the LPS-stimulated BV-2 cells. Scale bar, 20 µm. K TEM examined the ultrastructure of cells, × 10,000. Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: CTR-1 Abmart , T510261 AB_3644314 , Rabbit 1:1000.

Techniques: Disruption, Functional Assay, Comparison, Expressing, Knockdown, Control, Western Blot, Quantitative RT-PCR, Fluorescence

Journal: International Journal of Molecular Sciences

Article Title: Hsp70 and Calcitonin Receptor Protein in Extracellular Vesicles from Glioblastoma Multiforme: Biomarkers with Putative Roles in Carcinogenesis and Potential for Differentiating Tumor Types

doi: 10.3390/ijms25063415

Figure Lengend Snippet: Hsp70 and CTR are present in SEC-purified EVs from GBM patients. ( A , B ) Western blot and statistical analysis for Hsp70, and ( A , C ) for CTR (loading 50 μg, and we used Cyt C as a negative control). From the left: lane 1, EVs derived from the plasma of healthy individuals (HV); lane 2, EV lysates derived from the plasma of GBM patients at T0; lane 3, EV lysates derived from the plasma of GBM patients at T1. The images are representative of at least 3 independent experiments. ( D ) Representative images of immunohistochemistry for CTR in normal tissue (NT) ( a , b ), and GBM tissue (GBMT) ( c , d ). ( a , c ): Magnification, 200×; scale bar, 50 µm. ( b , d ): Magnification, 400×; scale bar, 20 µm. The histogram shows the statistical analysis of the IHC data (* p ≤ 0.01). ( E ) Immunofluorescence images of CTR showing its presence in the primary and secondary cell lines. A specific primary antibody for the receptor and a secondary antibody conjugated with FITC (green fluorescence) were used, and the cell nuclei (blue) were stained with DAPI. Magnification, 400×.

Article Snippet: Sections were then incubated with the primary antibody for CTR (mouse monoclonal, 31/01-1H10-4-1-14 clone, BioRad cat. N°: MCA2191; dilution 1:100).

Techniques: Purification, Western Blot, Negative Control, Derivative Assay, Clinical Proteomics, Immunohistochemistry, Immunofluorescence, Fluorescence, Staining

Journal: European Journal of Histochemistry : EJH

Article Title: Deletion of osteopontin in non-small cell lung cancer cells affects bone metabolism by regulating miR-34c/Notch1 axis: a clue to bone metastasis

doi: 10.4081/ejh.2023.3631

Figure Lengend Snippet: Deletion of osteopontin (OPN) inhibited the differentiation of osteoclasts. A ) RANK-positive rate of RAW264.7 cells was detected by flow cytometry. B ) The protein expressions of OCs markers Cathepsin K and CTR were detected by Western blot. C ) The area of F-actin ring was evaluated by immunofluorescence staining.

Article Snippet: The antibodies used in this study were purchased from Affinity Biosciences (Cincinnati, OH, USA), including anti-OPN (AF0227, 1:1000 dilution), anti-Cathepsin K (DF6614, 1:500 dilution), anti- CTR (DF10202, 1:2000 dilution), anti-RANKL (AF0313, 1:500 dilution), anti-M-CSF (DF12536, 1:300 dilution), anti-OPG (DF6824, 1:500 dilution), anti-Cleaved-Notch 1 (AF5307, 1:100 dilution), and anti-β-actin (AF7018, 1:1000 dilution).

Techniques: Flow Cytometry, Western Blot, Immunofluorescence, Staining